A NSW woman is in hospital and two Victorian men have been "affected" in a terrorist attack in the Spanish city of Barcelona that has been compared to Melbourne's Bourke Street Mall rampage.
Foreign Affairs Minister Julie Bishop said the woman was in a serious but stable condition. The exact condition of the men is unclear.
"They're in direct contact with consular staff. They have advised they were directly affected," Ms Bishop said in Melbourne on Friday.
"I'm not sure if they have injured or they just need to see a medical professional."
Consular officials were seeking more information on the injured NSW woman. Officials are also scouring hospitals in search of any other Australians.
"We know she is in a serious but stable condition. Our consular staff will be at the hospital as soon as possible."
More than 13 people were killed - Ms Bishop put the toll at 16 - and more than 100 injured when a white van ploughed into crowds on Las Ramblas.
Witnesses described horrific scenes and fearful crowds as the attack, which has been claimed by Islamic State, took place.
Ms Bishop described it as an "atrocity".
"The Australian government condemns this brutal and deliberate act clearly designed to harm tourists on holidays in Spain," she said.